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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/112656
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- Title
- Towards a Cryptosporidium proteome map and the search for a diagnostic
- Related
- Australian Young Water Professionals Conference (1st : 2006) (15 - 17 February 2006 : Sydney)
- Related
- Storey, M. V. and Le-Clech, P.. Conference proceedings : 1st Australian Young Water Professionals Conference, The Scientia, UNSW Sydney, February 15th-17th 2006
- Related
- http://www.cwwt.unsw.edu.au/ywp2006/papers/YWP%203.8.pdf
- Publisher
- Sydney : Conference Organising Committee
- Date
- 2006
- Author/Creator
- Truong, Q
- Author/Creator
- Ferrari, B. C
- Description
- The 1998 Sydney Water Crisis and the Milwaukee cryptosporidiosis outbreak in 1993 have highlighted the need for a more specific diagnostic target against Cryptosporidium. Sydney experienced no reported infections, whilst Milwaukee resulted in many infections, some resulting in mortalities. In both cases, economic losses were in the millions of dollars and public health confidence was compromised. Malarial studies have demonstrated that the location and modifications of parasite proteins are areas that need to be addressed in understanding disease processes and genomics can not provide these answers. Proteomic analysis by 2D-PAGE is one such technology that could be employed. By employing 2D-PAGE, we aim to construct a proteome map of Cryptosporidium which will then be applied to identify novel proteins for antibody development. Prior to proteomic analysis, Cryptosporidium oocysts need to be purified from calf faecal samples and excysted. Qualitative assurance is required in both procedures prior to proteomics. This paper describes the developmental stages involved prior and during 2D-PAGE analysis in our search for a more targeted diagnostic.
- Description
- 6 page(s)
- Subject Keyword
- Cryptosporidium
- Subject Keyword
- flow cytometry
- Subject Keyword
- proteomics
- Subject Keyword
- purification
- Subject Keyword
- 2D-PAGE
- Resource Type
- journal article
- Organisation
- Macquarie University. Dept. of Chemistry and Biomolecular Sciences
- Identifier
- http://hdl.handle.net/1959.14/112656
- Identifier
- mq:12000
- Identifier
- ISBN:0646457551
- Identifier
- mq-rm-2013014651
- Identifier
- mq-rm-2006005286
- Language
- eng
Macquarie University ResearchOnline
