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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/202465
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- Title
- Fabrication and application of rose bengal-chitosan films in laser tissue repair
- Related
- Journal of visualized experiments, No. 68, (2012),
- DOI
- 10.3791/4158
- Publisher
- Journal of Visualized Experiments
- Date
- 2012
- Author/Creator
- Lauto, Antonio
- Author/Creator
- Stoodley, Marcus
- Author/Creator
- Barton, Matthew
- Author/Creator
- Morley, John W
- Author/Creator
- Mahns, David A
- Author/Creator
- Longo, Leonardo
- Author/Creator
- Mawad, Damia
- Description
- (Associated video file can be found at: http://dx.doi.org/10.3791/4158) Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two tissue edges¹,². These are then irradiated by a laser that is selectively absorbed by the RB. The resulting photochemical reactions supposedly crosslink the collagen fibers in the tissue with minimal heat production³. In this report, RB has been incorporated in thin chitosan films to fabricate a novel tissue adhesive that is laser-activated. Adhesive films, based on chitosan and containing ~0.1 wt% RB, are fabricated and bonded to calf intestine and rat tibial nerves by a solid state laser (λ=532 nm, Fluence~110 J/cm², spot size~0.5 cm). A single-column tensiometer, interfaced with a personal computer, is used to test the bonding strength. The RB-chitosan adhesive bonds firmly to the intestine with a strength of 15 ± 6 kPa, (n=30). The adhesion strength drops to 2 ± 2 kPa (n=30) when the laser is not applied to the adhesive. The anastomosis of tibial nerves can be also completed without the use of sutures. A novel chitosan adhesive has been fabricated that bonds photochemically to tissue and does not require sutures.
- Description
- 5 page(s)
- Subject Keyword
- 060800 Zoology
- Subject Keyword
- Photochemical tissue bonding
- Subject Keyword
- Tissue repair
- Subject Keyword
- Nerve anastomosis
- Subject Keyword
- Sutureless technique
- Subject Keyword
- Chitosan
- Subject Keyword
- Surgical adhesive
- Resource Type
- journal article
- Organisation
- Macquarie University. Australian School of Advanced Medicine
- Identifier
- http://hdl.handle.net/1959.14/202465
- Identifier
- mq:23711
- Identifier
- ISSN:1940-087X
- Identifier
- mq-rm-2011011040
- Identifier
- mq_res-ext-2-s2.0-84868286360
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
