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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/282759
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- Title
- Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes
- Related
- Journal of biomedical optics, Vol. 18, No. 7, (2013), p.076004-1-076004-10
- DOI
- 10.1117/1.JBO.18.7.076004
- Publisher
- SPIE
- Date
- 2013
- Author/Creator
- Grebenik, Ekaterina A
- Author/Creator
- Nadort, Annemarie
- Author/Creator
- Zubov, Vitali P
- Author/Creator
- Klinov, Dmitry V
- Author/Creator
- Panchenko, Vladislav Y
- Author/Creator
- Deyev, Sergey M
- Author/Creator
- Zvyagin, Andrei V
- Author/Creator
- Generalova, Alla N
- Author/Creator
- Nechaev, Andrei V
- Author/Creator
- Sreenivasan, Varun K. A
- Author/Creator
- Khaydukov, Evgeny V
- Author/Creator
- Semchishen, Vladimir A
- Author/Creator
- Popov, Alexey P
- Author/Creator
- Sokolov, Viktor I
- Author/Creator
- Akhmanov, Aleksandr S
- Description
- Innovative luminescent nanomaterials, termed upconversion nanoparticles (UCNPs), have demonstrated considerable promise as molecular probes for high-contrast optical imaging in cells and small animals. The feasibility study of optical diagnostics in humans is reported here based on experimental and theoretical modeling of optical imaging of an UCNP-labeled breast cancer lesion. UCNPs synthesized in-house were surface-capped with an amphiphilic polymer to achieve good colloidal stability in aqueous buffer solutions. The scFv4D5 mini-antibodies were grafted onto the UCNPs via a high-affinity molecular linker barstar: barnase (Bs:Bn) to allow their specific binding to the human epidermal growth factor receptor HER2/neu, which is overexpressed in human breast adenocarcinoma cells SK-BR-3. UCNP-Bs:Bn-scFv4D5 biocomplexes exhibited high-specific immobilization on the SK-BR-3 cells with the optical contrast as high as 10:1 benchmarked against a negative control cell line. Breast cancer optical diagnostics was experimentally modeled by means of epi-luminescence imaging of a monolayer of the UCNP-labeled SK-BR-3 cells buried under a breast tissue mimicking optical phantom. The experimental results were analyzed theoretically and projected to in vivo detection of early-stage breast cancer. The model predicts that the UCNP-assisted cancer detection is feasible up to 4 mm in tissue depth showing considerable potential for diagnostic and image-guided surgery applications.
- Description
- 10 page(s)
- Subject Keyword
- 111200 Oncology and Carcinogenesis
- Subject Keyword
- upconversion nanoparticle
- Subject Keyword
- breast cancer
- Subject Keyword
- tissue phantom
- Subject Keyword
- human epidermal growth factor receptor 2
- Subject Keyword
- mini-antibody (wild type)
- Subject Keyword
- biomedical optical imaging
- Resource Type
- journal article
- Organisation
- Macquarie University. Department of Physics and Astronomy
- Organisation
- Macquarie University. MQ BioFocus Research Centre
- Identifier
- http://hdl.handle.net/1959.14/282759
- Identifier
- mq:30206
- Identifier
- ISSN:1083-3668
- Identifier
- mq-rm-2013001572
- Identifier
- mq_res-ext-wos323030400012
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
