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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.14/1152610
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- Title
- Measurement of Lipid Hydroperoxides by the Ferric-Xylenol Orange Method (1) Characteristics of the Ferric-Xylenol Orange/Membrane Phosphatidylcholine Complex
- Related
- Journal of Nutritional Science and Vitaminology, Vol. 55, No. 1, (2009), p.9-14
- DOI
- 10.3177/jnsv.55.9
- Publisher
- Center for Academic Publications Japan
- Date
- 2009
- Author/Creator
- Fukuzawa, Kenji
- Author/Creator
- Shibata, Akira
- Author/Creator
- Okamura, Chika
- Author/Creator
- Fujiwara, Yuhki
- Author/Creator
- Akai, Kaori
- Author/Creator
- Tsuchiya, Koichiro
- Author/Creator
- Tokumura, Akira
- Author/Creator
- Gebicki, Janusz
- Description
- The ferric-xylenol orange (FOX) method for measurement of hydroperoxides is based on a technique that employs reduction of peroxides in an acidic condition by Fe2+ and formation of the colored ferric-xylenol orange (XO/Fe3) product with a peak at 560 nm. The 560 nm absorbance peak of XO/Fe3+ shifts to a 610 nm peak with high absorption intensity in the presence of phosphatidylcholine. This is useful for quantification of peroxides such as phospholipid hydroperoxides. Based on this finding, we recently reported a modified FOX method. We now show by measurements of absorbance, broadening of the electron paramagnetic resonance spectrum, changes in the vesicle size and their zeta potentials, the effects of detergents, and manipulation of the membrane lipid composition that the XO/Fe3 -phosphatidylcholine complex forms only in the presence of intact phosphatidylcholine membranes. The phosphate group on the phospholipid plays a role in this interaction which may involve an electron transfer from the phosphate to the Fe3+. A positively charged quaternary amine on the phosphatidylcholine is also necessary to give a peak absorbance at 610 nm. Our observations are consistent with binding of one X0/Fe3+ COmplex to about 3 molecules of the egg yolk phosphatidylcholine carrying a zero net charge.
- Description
- 6 page(s)
- Subject Keyword
- 111100 Nutrition and Dietetics
- Subject Keyword
- Lipid peroxide
- Subject Keyword
- Liposome
- Subject Keyword
- Membrane
- Subject Keyword
- Phosphatidylcholine
- Subject Keyword
- Xylenol orange
- Resource Type
- journal article
- Organisation
- Macquarie University. Department of Biological Sciences
- Identifier
- http://hdl.handle.net/1959.14/1152610
- Identifier
- mq:55869
- Identifier
- ISSN:0301-4800
- Identifier
- mq-rm-2009004176
- Language
- eng
- Reviewed
Macquarie University ResearchOnline
