In recent years the use of mindfulness training as a therapeutic intervention for a variety of clinical conditions has been increasing. The Five Facet Mindfulness Questionnaire (FFMQ, Baer, Smith, Hopkins, Krietemeyer, & Toney, 2006) is the most comprehensive mindfulness measure to date and is likely to be widely utilised in mindfulness research. However, doubts exist surrounding the factor struture of the FFMQ, especially given that the FFMQ's development sample was comprised entirely of udnergraduate students and included few experienced meditators. To address this, the current study assessed a heterogeneous sample of meditators and non-meditators to test the fit of the FFMQ's hierarchical five-factor model. A total of 461 respondents completed a web-based survey (mean age = 36.5, SD = 14.3; 312 females, 149 males). Over half of the combined community and university student sample in the present study (52.7%) consisted of beginners and very experienced meditators, including members of a large number of Buddhist meditation groups and mindfulness interest groups both in Australia and the UK. A confirmatory factor analysis supported the FFMQ's hierarchical five-factor structure. This supports the hypothesis that the lack of fit found in the study by Baer et al. (2006) was due to a lack of experienced meditators in the development sample. The present study demonstrates the importance of utilising appropriate samples when asking a specific research question.